ALZHEIMER’S DISEASE: IDENTIFIED ONE UPREGULATED GLYCOPROTEIN
Huang and colleagues are studying all the main brain glycoproteins and their preliminary results were recently reported in the European Journal of Neuroscience (Elevation in the level and activity of acid ceramidase in Alzheimer’s disease brain. Eur. J. Neurosci 20, 3489-3497, 2004).
They used affinity chromatography to isolate glycoproteins from post-mortem cerebral cortical tissue. The glycoproteins were separated using SDS-polyacrilamide gel electrophoresis and analysed by mass spectrometry. Eleven glycoproteins were isolated and only one showed increased expression levels and activity in the brains of individuals who suffered from Alzheimer’s disease: the β-subunit of the lysosomal enzyme acid ceramidase.
The enzyme showed marked co-localization with the intracellular neurofibrillary tangles.